Composite

Part:BBa_K5102072:Design

Designed by: Natalia Kuźmierkiewicz   Group: iGEM24_Munich   (2024-09-30)


pRAM_ProgRAM-recording-tape1.0


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 18
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 18
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2494
    Illegal BamHI site found at 3554
    Illegal XhoI site found at 2563
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 18
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 18
    Illegal NgoMIV site found at 4899
    Illegal AgeI site found at 3645
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The composite part consists of: CMV enhancer, CMV promotor, T7 promoter, recording tape 1.0, T2A, miRFP670nano3, P2A, eUnaG, T2A, mScarlet3, P2A, eUnaG, T2A, mTagBFP2, P2A, eUnaG, human beta-globin 3'UTR, PP7, T7 terminator, WPRE, SV40 polyA.

Source

The composite was assembled from gblocks and oligos provided by a DNA synthesis provider.

References

Truong, D.-J. J. et al. Exonuclease-enhanced prime editors. Nat Methods 21, 455–464 (2024).